E. Genital and Anal evidence 1. Collect matted portion of the pubic hair of SAS by cutting from the base, air-dry and seal it in an envelope. 2. Two swabs each should be taken from the vulva, vagina and anal opening. Two slides for each swab should be made, air dried and packed. 3. Two vaginal smears should be prepared on the glass slides, air-dried and sent for seminal fluid/ spermatozoa examination. 4. Vaginal washing should be collected using a sterilised syringe and a small sterilised rubber catheter. 2-3 ml of saline should be instilled in the vagina and fluid should be aspirated. Fluid filled syringe should be sent to the FSL after putting a knot over the rubber catheter. F. Handing over samples to FSL 1. For handing over the samples, a requisition letter should be addressed to the concerned FSL stating what all samples are being sent and what each sample needs to be tested for. 2. This requisition letter must be signed by the examining doctor and the police personnel to whom the sample has been handed over shall also sign the letter. 3. Requisition letter should be duly-checked to ensure that the numbering of individual packets is in accordance with the numbering on the requisition form. G. General 1. It is very important to air dry all the samples before sealing them as this is the only way to prevent its degradation. 2. The samples should also be labelled properly. 3. The swab sticks used for collection of samples should be moistened with distilled water.